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F1000 Factor 9.7

Two-photon imaging of lymphocyte motility and antigen response in intact lymph node.
Miller MJ, Wei SH, Parker I, Cahalan MD
Science 2002 Jun 7 296(5574):1869-73 [abstract on PubMed] [related articles] [order article]
Selected by | Casey Weaver / Jonathan Howard / Matthias von Herrath
First evaluation 14 Jun 2002 | Latest evaluation 1 Jul 2002
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Faculty Comments
Faculty Member Comments
Casey Weaver
University of Alabama at Birmingham, United States of America

New Finding
Tech Advance
This study introduces two photon-videomicroscopy to the study of T cell and B cell motility, and T cell responses to specific antigen, in explanted lymph nodes. Striking differences in the motilities of T and B cells within the lymph node architecture are directly observed. The kinetics and character of the response of T cells to specific antigen are defined.

Evaluated 1 Jul 2002
Jonathan Howard
Institut fur Genetik, Germany

Tech Advance
Modern microscopic methods, especially two-photon laser scanning photography, allow direct visualisation of live fluorochrome-labelled lymphocytes trafficking in lymphoid tissues. Differentially labelled T and B cells move about in complex trajectories after entering their characteristic regions of a lymph node. The presence of an antigen specific for labelled T lymphocytes induces perturbations in their movement. In particular, stationary clusters and dynamic swarms form. Subsequently, the cells enlarge and divide. Without breaking radical new ground conceptually, these results demonstrate the validity of concepts of lymphocyte behaviour obtained with disaggregated cell mixtures. They further demonstrate the extraordinary power of the new microscopy.

Evaluated 26 Jun 2002
Matthias von Herrath
La Jolla Institute for Allergy and Immunology, United States of America

Tech Advance
This is a very impressive advance for the live tracking of ongoing antigen-specific immune responses, and could be a first step in revolutionizing non-invasive imaging techniques for understanding immunity. Direct visualization of antigen-specific T cells and antigen-presenting cells in lymph nodes and possibly other organs in the future will improve our comprehension of the immune system.

Evaluated 14 Jun 2002
Faculty Comments

How to cite the Faculty of 1000 Biology evaluation(s) for this paper

1) To cite all the evaluations for this article:

Faculty of 1000 Biology: evaluations for Miller MJ et al Science 2002 Jun 7 296 (5574) :1869-73 http://www.facultyof1000.com/article/12016203/evaluation

2) To cite an evaluation by a specific Faculty member:

Casey Weaver: Faculty of 1000 Biology, 1 Jul 2002 http://www.facultyof1000.com/article/12016203/evaluation

Jonathan Howard: Faculty of 1000 Biology, 26 Jun 2002 http://www.facultyof1000.com/article/12016203/evaluation

Matthias von Herrath: Faculty of 1000 Biology, 14 Jun 2002 http://www.facultyof1000.com/article/12016203/evaluation

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